BUILDING A PHYLOGENETIC TREE FOR QUÝT HOI (CITRUS AURANTIUM) BASED ON THE matK GENE SEQUENCE
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Abstract
Molecular markers, particularly DNA barcodes, are widely applied in species identification and conservation due to their high reliability and independence from morphological traits. In this study, we isolated and sequenced a fragment of the maturase K (matK) gene from Citrus aurantium (Quýt hoi) to evaluate its genetic characteristics and phylogenetic relationships. The sequencing results showed that the matK gene fragment was 837 bp in length. Comparative sequence analyses with reference sequences available in the GenBank confirmed that the studied sample exhibited noticeable genetic divergence from other reported Citrus species. This finding suggests that the species may represent a distinct variant or an independently evolved form, highlighting its potential for molecular taxonomy and breeding research.
Keywords
DNA barcode, Citrus aurantium, sequence gene matK, genetic relationship.
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References
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