NGHIÊN CỨU ẢNH HƯỞNG CỦA MỘT SỐ CHẮT KÍCH THÍCH SINH TRƯỞNG ĐÉN KHÁ NANG NHÂN NHANH CHỒI KEO LAI DÒNG BV75 TRONG NHẨN GIỐNG BẰNG PHƯƠNG PHÁP IN VITRO

Abstract

With the outstanding advantages o f Acacia hybrid compared to other forest species, this species currently occupies a large part o f Vietnam's afforestation area. However, propagation by traditional methods such as cuttings and seeding does not meet the demand fo r disease-free seedlings, uniform in quality. Breeding technology^ by tissue culture method Is considered the leading technology solution to maintain the genetic quality o f seedlings. Plant Growth Regulators are an important factor in increasing shoot multiplier. Cytokinin N6-benzyladenine (BAP) has the ability to stimulate the Acacia hybrid BV75subspecies shoot buds with high shoot multiplier (7.7 shoots/clump, shoot multiplier is 2.96 times) at 1.5mg/L in MS * media. When using Cytokinin and Auxin combination, the most suitable combination for multiplication is 1.5mg/L BAP and 1.0mg/L NAA ỉn MS * medium (7.8 buds/clump and multiplier buds 3.0 times, bud height is 4.5 cm, buds grow well). This is also the environment for the best quality shoots to serve the next phase of research, rooting.
Keywords: Acacia hybrid, in vitro method, plant growth regulator, cytokinin, auxin.