ASSESSMENT OF THE PRESENCE OF SOME SILVER LEAF
RESISTANCE GENES (XA5, XA7, XA13, XA21) IN KBL2 RICE VARIETY

Quang Tin Nguyen, Thi Minh Nguyet Nguyen, Thi Lan Nguyen

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Abstract

The results of research have identified the presence of some rice silver leaf resistance genes in the KBL2 rice variety. Assessment of the presence was conducted among 100 individuals of the KBL2 rice variety included in the evaluation of which individuals 5, 21, 67 and 72 carry the xa5 resistance gene in the homozygous state, 4 individuals 9, 51, 66 and 74 carry the xa5 resistance gene in the heterozygous state, 1 individual number 45 carries the Xa21 gene in the homozygous state and only individual number 88 carries the two resistance genes xa5 and Xa21 in the homozygous state. The presence of resistance genes Xa7 and xa13 was not detected in the individuals included in the assessment.

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References

[1] Furuya N, Taura S, Goto T, Thuy BT, Ton PH, Tsuychia K and Yoshimura A (2012), Diversity in Virulence of Xanthomonas oryzae pv, oryzae from Northern Vietnam, JARQ, 46 (4), 329 - 338.
[2] Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush GS (1997), Pyramiding of bacterial blight resistance genes in rice: marker assisted selection using RFLP and PCR, Theor Appl Genet, 95: 313-320.
[3] INGER Genetic Resources Center (2002), Standard evaluation system for rice. 4th ed. International Rice Research Institute. P.O. Box 933, 1099 Manila, Philippines.
[4] Wang S., Luo Y., Li C., Wu S., Wang D., Du S. (2004), Improvement of Resistance to Bacterial Blight by Marker-Assisted Selection in a Wide Compatibility Restorer Line of Hybrid Rice, Rice Science, 11(5-6): 231-237.
[5] Plan Breeding, Genetics and Biotechnology Division (2011), Protocol on Evaluation of Rice for Bacterial Blight Resistance Disease Resistance BB version 6A. International Rice Research Institute. P.O. Box 933, 1099 Manila, Philippines.
[6] Raman M.S., Mamme R.V., Sunil K.B., Gouri SL., Gajjala AR., Rani NS., Nukula PS., Ramesh VS. (2008). Marker assisted introgression of bacterial blight resistance in Samba Mahsuri, an elite indica rice variety, Euphytica, 160: 411-422.
[7] Wang HM Qi and Cutler AJ (1993), A simple method of preparing plant samples for PCR, Nucleic Acids Res., 21(17): 4153-4154.