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DOI: 10.70117/hdujs.81.11.2025.1088
Issue
Số 81-11.2025: Khoa học Nông - Lâm - Ngư nghiệp
Section
Khoa học Nông - Lâm - Ngư nghiệp
How to Cite
Nguyen, V. H., Le, D. C., & Le, T. L. (2025). Xây dựng cơ cở dữ liệu barcode rpoB cho cây Mật gấu (Vernonia amygdalina Del.) tại Thanh Hóa. Hong Duc University Journal of Science, 81(11). https://doi.org/10.70117/hdujs.81.11.2025.1088

Xây dựng cơ cở dữ liệu barcode rpoB cho cây Mật gấu (Vernonia amygdalina Del.) tại Thanh Hóa

Nguyễn Việt Hải1, TS. Lê Đình Chắc , Lê Thị Lâm2
1 Trung tâm nghiên cứu dược liệu Bắc Trung bộ, Viện dược liệu
2 Hong Duc University

Main Article Content

Abstract

Abstract

Molecular markers (DNA barcodes) used for identifying medicinal plant species are considered highly reliable and widely applicable, particularly for taxa of pharmacological importance. Accordingly, DNA barcoding has become an indispensable tool in taxonomic research and conservation. Fundamentally, a DNA barcode is a DNA sequence of approximately 400-1000 bp that serves as a molecular marker for rapidly and accurately determining phylogenetic relationships and species identity, independent of morphological characteristics of specimens. In this study, we isolated and sequenced the rpoB gene of Vernonia amygdalina Del. (commonly known as bitter leaf) and assessed its genetic relationships as a basis for subsequent investigations. The amplified rpoB fragment was 506 bp in length and was used for comparative analysis against published rpoB sequences in GenBank. The results showed that the rpoB sequence of our sample formed a completely distinct genetic lineage, separate from all previously published rpoB sequences. This finding demonstrates that the rpoB sequence of the studied specimens exhibits substantial genetic divergence relative to GenBank data. These results support the reliability and accuracy of using the rpoB gene fragment to elucidate the genetic relationships of Vernonia amygdalina Del.

Keywords

DNA barcode, Mật gấu, đoạn gene rpoB, Mã vạch DNA.

Article Details

References

[1] Allen, G. C, Flores-Vergara M. A, Krasynanski. S, Kumar. S, and Thompson W. F, (2006) “A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide,” Nat Protoc., vol. 1, pp. 2320-2325.
[2] Farombi, E.O. & Owoeye, O. (2011). Phytochemistry and medicinal properties of Vernonia amygdalina. Journal of Medicinal Food, 14(12), 1310–1316.
[3] Hollingsworth, P. M., Forrest, L. L., Spouge, J. L., Hajibabaei, M., Ratnasingham, S., van der Bank, M., … Little, D. P. (2009), “A DNA barcode for land plants”, Proceedings of the National Academy of Sciences of the United States of America, 106(31), 12794-12797. https://doi.org/10.1073/pnas.0905845106
[4] Mark W. Chase; Nicolas Salamin; Mike Wilkinson; James M. Dunwell; Rao Prasad Kesanakurthi; Nadia Haider; Vincent Savolainen, (2005). Land plants and DNA barcodes: Short-term and long-term goals. Philosophical Transactions of the Royal Society B, 360, 1889-1895.
[5] M. Stoeckle, (2003) “Taxonomy, DNA, and the Bar Code of Life,” BioScience, vol. 53, no. 9, pp.796-797.
[6] Kress, W. J., & Erickson, D. L. (2007). A two-locus global DNA barcode for land plants: The coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE, 2(6), e508.
[7] S. Kumar, G. Stecher, M. Sulesky, M. Sanderford, S. Sharma, and K. Tamura, “MEGA12: Molecular Evolutionary Genetic Analysis Version 12 for Adaptive and Green Computing,” Molecular Biology and Evolution, vol. 41, no. 12, msae263, 2024.
[8] Yeap SK, Ho WY, Beh BK, Ng KT, Abdul AB, Syamsumir DF, Koh SP, Thomas NF, and Husain K. (2010). Anticancer and immunomodulatory properties of Vernonia amygdalina. Pharmaceutical Biology, 48(11), 1162–1169.