Using the DNA barcode markers to identification Croton kongensis gagnep. species in Thanh Hoa
Main Article Content
Abstract
Using the DNA barcode markers to identification Croton kongensis gagnep. species in Thanh Hoa
Abstract
Croton kongensis Gagnep. is a valuable medicinal plant that contains many bioactive compounds with high pharmacological value. As a result, this species is widely used in traditional medicine. Currently, this species has been identified as one of the medicinal plants containing bioactive compounds capable of combating certain diseases such as cancer, cardiovascular diseases, and digestive disorders. Therefore, the use of DNA barcoding to identify Croton kongensis in particular, and medicinal plants in general, has been the focus of many researchers both domestically and internationally. DNA barcoding is a technique that uses a specific DNA sequence (approximately 400–1000 bp) as a marker to determine phylogenetic relationships and identify species quickly and accurately. This approach not only enables pharmacologists to rapidly and accurately identify medicinal plants but also helps in detecting counterfeit medicinal species or rare species that need conservation.
In this study, we focus on identifying Croton kongensis Gagnep. using the trnH DNA barcode sequence. The results of sequencing and analyzing the trnH region revealed a sequence length of 430 bp, confirming its identity as Croton kongensis, providing a foundation for further research.
Keywords
DNA barcode, Croton sp., gene trnH, trnH.
Article Details
References
1. Bộ Y tế, Dược điển Việt Nam V (2018), tập 2. Nhà xuất bản Y học.
2. Đỗ Tất Lợi, Cây thuốc Việt Nam (2006). Nhà xuất bản Y học.
3. G. C. Allen, M. A. Flores-Vergara, S. Krasynanski, S. Kumar, and W. F. Thompson, (2006) “A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide,” Nat Protoc., vol. 1, pp. 2320-2325.
4. K. Tamura, G. Stecher, and S. Kumar, (2021) “MEGA11: Molecular Evolutionary Genetics Analysis version 11,” Molecular Biology and Evolution, vol. 38, pp. 3022-3027.
5. Kress WJ, Wurdack JK, Zimmer EA, Weigt LA, Janzen DH: Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci USA. 2005, 102: 8369-8374. 10.1073/pnas.0503123102.
6. L. Sun, Z. Meng, Z. Li, B. Yang, Z. Wang, G. Ding, and W. Xiao, (2014) “Two new natural products from Croton kongensis Gagnep,” Nat Prod Res, vol. 28, no. 8, pp. 563-567.
7. M. J. Wang, M. Wang, X. Q. Zhan, L. Liu, Q. Wu, F. L. An, Y. B. Lu, L. L. Guo, Z. X. Zhang , and D. Q. Fei, (2023) “Antimicrobial diterpenoids from the leaves and twigs of Croton kongensis Gagnepain,” Fitoterapia, vol. 164, Art. no. 105350.
8. M. Stoeckle, (2003) “Taxonomy, DNA, and the Bar Code of Life,” BioScience, vol. 53, no. 9, pp.796-797.
9. R. Z. Fan , L. Chen , T. Su , W. Li , J. L. Huang , J. Sang , G. H. Tang , and S. Yin, (2021) “Discovery of 8,9- seco- ent-Kaurane Diterpenoids as Potential Leads for the Treatment of Triple-Negative Breast Cancer,” J Med Chem, vol. 22, no. 64(14), pp. 9926-9942.
10. Sambrook, J., Fritsch, E. R., & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
11. T. T. Dao, K. Y. Lee, H. M. Jeong, P. H. Nguyen, T. L. Tran, P. T. Thuong, B. T. Nguyen, and W. K. Oh, (2011) “ent-Kaurane diterpenoids from Croton tonkinensis stimulate osteoblast differentiation,” J Nat Prod., vol. 74, no. 12, pp. 2526-2531.
12. Y. Y. Fan, S. Q. Shi, G. Z. Deng, H. C. Liu, C. H. Xu, J. Ding, G. W. Wang, and J. M. Yue, (2020) “Crokonoids A-C, A Highly Rearranged and Dual-Bridged Spiro Diterpenoid and Two Other Diterpenoids from Croton kongensis,” Org Lett, vol. 7, no. 22(3), pp. 929-933.