ISOLATION AND IDENTIFICATION OF THE FUNGUS ASPERGILLUS ORYZAE USED IN FERMENTED RICE MILK PRODUCTION
Main Article Content
Abstract
Aspergillus oryzae is a fungus widely used in food industry for manufacturing fermented foods due to health beneficial properties and safety. Isolation and identification of A. oryzae plays an important role in making microbial inoculants for fermentation processes. The characterization of the isolated fungus was carried out through morphological observations and the amplification and sequencing of DNA fragments (ITS regions), achieving the identification of Aspergillus oryzae.
Keywords
Aspergillus oryzae, fermentation food, fungus identification.
Article Details
References
[1] Charles Thom, Margaret B. Church (1921), Aspergillus flavus, A. oryzae and asscociated species. American Journal of Botany, Feb., 8(2):103-126.
[2] Daniel K. Manter and Jorge M. Vivanco (2007), Use of the ITS primers, ITS1F and ITS4, to characterize fungal abundance and diversity in mixed-template samples by qPCR and length heterogeneity analysis, Journal of Microbiological Methods, (71):7-14, Doi:10.1016/j.mimet.2007.06.016.
[3] Elkhateeb, W.A., (2005), Some mycological, phytopathological and physiological studies on mycobiota of selected newly reclaimed soils in Assiut Governorate, Egypt (M. Sc. Thesis, Faculty of Science, Assuit University, Egypt).
[4] Hinrikson H.P, Hurst S.F, Morrison C.J (2005), Molecular methods for the identification of Aspergillus species. Medical Mycology Supplement 1, (43):129-137.
[5] Machida M (2002), Progress of Aspergillus oryzae genomics, Adv Appl Microbiol (51):81-106.
[6] Moubasher AH (1993), Soil fungi in Qatar and other Arab countries, The Scientific And Applied Research Centre University of Qatar, Doha.
[7] Yoshio Otani (1939), Some relations between morphology and physiology of Aspergillus oryzae, The zymomycological Institute, the imperial college of agriculture, Tottori, Japan, 15(3):33-36.
[8] Watarai N, Yamamoto N, Sawada K, Yamada T (2019), Evolution of Aspergillus oryzae before and after domestication inferred by large-scale comparative genomic analysis, DNA Res, 26(6):465-472.
[2] Daniel K. Manter and Jorge M. Vivanco (2007), Use of the ITS primers, ITS1F and ITS4, to characterize fungal abundance and diversity in mixed-template samples by qPCR and length heterogeneity analysis, Journal of Microbiological Methods, (71):7-14, Doi:10.1016/j.mimet.2007.06.016.
[3] Elkhateeb, W.A., (2005), Some mycological, phytopathological and physiological studies on mycobiota of selected newly reclaimed soils in Assiut Governorate, Egypt (M. Sc. Thesis, Faculty of Science, Assuit University, Egypt).
[4] Hinrikson H.P, Hurst S.F, Morrison C.J (2005), Molecular methods for the identification of Aspergillus species. Medical Mycology Supplement 1, (43):129-137.
[5] Machida M (2002), Progress of Aspergillus oryzae genomics, Adv Appl Microbiol (51):81-106.
[6] Moubasher AH (1993), Soil fungi in Qatar and other Arab countries, The Scientific And Applied Research Centre University of Qatar, Doha.
[7] Yoshio Otani (1939), Some relations between morphology and physiology of Aspergillus oryzae, The zymomycological Institute, the imperial college of agriculture, Tottori, Japan, 15(3):33-36.
[8] Watarai N, Yamamoto N, Sawada K, Yamada T (2019), Evolution of Aspergillus oryzae before and after domestication inferred by large-scale comparative genomic analysis, DNA Res, 26(6):465-472.